Lentiviral-based miRNA overexpression library

Enabling high-throughput cell-based screening to systematically address the biological function of miRNAs, we constructed and validated a lentiviral miRNA expression library containing annotated and candidate human miRNA precursors. We demonstrated its functionality in both short- and long-term assays with focus on interference in cancer pathways, cell proliferation and death (apoptosis), epithelial-to-mesenchymal transition, invasion and migration, and angiogenesis.