InteRNA’s platform provides competitive edge in target selection
InteRNA Technologies created the core of its technology platform using massively parallel sequencing and proprietary, highly advanced bioinformatics methods to analyze, predict, and validate miRNA sequences. This has led to the identification of a large number of novel miRNAs. InteRNA further analyzed an extensive collection of small RNAs from different human tissues to refine existing annotations and establish miRNA expression patterns. Comparison of miRNA cloning frequencies in the different tissues allowed for identification of miRNA sets predominantly expressed in specific tissues, as well as arm-switching events and differential isomiR expression.
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- Berezikov, E., Thuemmler, F., van Laake, L., Kondova, I., Bontrop, R., Cuppen, E. and Plasterk, R. H. (2006). Diversity of microRNAs in human and chimpanzee brain. Nat. Genet., 2006, 38: 1375-1377
- Takada, S., Berezikov, E., Yamashita, Y., Lagos-Quintana, M., Kloosterman, W. P., Enomoto, M., Hatanaka, H., Fujiwara, S., Watanabe, H., Soda, M., Choi, Y. L., Plasterk, R. H. A., Cuppen, E. and Mano, H. (2006). Mouse microRNA profiles determined with a new and sensitive cloning method. Nucleic Acids Res., 2006, 34:e115
- Berezikov, E., Guryev, V., van de Belt, J., Wienholds, E., Plasterk, R. H. and Cuppen, E. (2005). Phylogenetic shadowing and computational identification of human microRNA genes Cell, 2005, 120: 21-24