Identification mode of action & biomarkers
InteRNA’s platform to identify the mRNAs regulated by the miRNA therapeutic drug candidates comprises RISC immunoprecipitation and RNAseq analysis of cells upon miRNA overexpression (identifying downregulated mRNAs). We correlate these datasets with those generated by target prediction and pathway analysis software tools and literature. Identified downstream-regulated mRNAs are subsequently confirmed by 3′-UTR assays, qPCR, ISH/IHC and Western blotting.
The regulated mRNAs serve as biomarkers for further development of the therapeutic miRNA drug candidates.